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KMID : 1140120080130010062
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Cancer Prevention Research
2008 Volume.13 No. 1 p.62 ~ p.67
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In Vitro Anticancer Effect of Chungkukjangs from Folk Villages of Sunchang Region in HT-29 Human Colon Cancer Cells
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Ryu Ki-Jung
Zhao Xin
Bak Soon-Sun
Kim Boh-Kyung
Jeon Jung-Tae
Park Kun-Young
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Abstract
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Six different chungkukjang products (K-, M-, Mn-, O-, Os-, H-chungkukjangs) were purchased from Folk Villages of Sunchang region, and 3 samples, K-, H- and M-chungkukjangs showed higher sensory evaluation and good fermented quality among the samples. The three chungkukjang samples showed in vitro anticancer activities in HT-29 human colon cancer cells. The anticancer effect of the 3 samples all showed same trends, however K-chungkukjang exhibited the highest anticancer activities in the HT-29 cells. MTT assay, growth inhibition tests and apoptosis analysis by DAPI staining and RT-PCR employed to check the anticancer effect in the colon cancer cells. At the concentration of 1.0 mg/ml, K-chungkukjang (86%) showed the highest growth inhibitory effect in the cancer cells, followed by H-chungkukjang (76%) and M-chungkukjang (44%) (p＜0.05). K-chungkukjang induced apoptosis as determined by DAPI staining and exhibited the increased Bax and decreased Bcl-2 in mRNA expression. Among the chungkukjang samples, K-chungkukjang showed the highest anticancer effects. It is concluded that chungkukjangs revealed anticancer activities, but the activity exhibited differently depending on the different product, probably due to the preparation methods. (Cancer Prev Res 13, 62-67, 2008)
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KEYWORD
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Sunchang region, Chungkukjang, HT-29 human colon carcinoma cells, Anticancer
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